| General description | The clones are sequenced-verified shRNA lentiviral plasmids (pLKO.1-puro) provided as frozen bacterial glycerol stocks (Terrific Broth, carbenicillin at 100 μg/ml and 10% glycerol). The set comes in 96-well plates that are barcoded for simple identification. A CD containing RefSeq, gene description, gene symbol, clone ID, hairpin sequence, locus link, and plate map positions are provided with the gene family set. |
| Other Notes | Number of Genes: 117, Number of Clones: 578 |
| | For a detailed listing of other available gene family sets, visit the gene family set website. |
| | Each MISSION shRNA clone is constructed within the lentivirus plasmid vector, pLKO.1-Puro, followed by transformation into Escherichia coli. The pLKO.1-Puro vector contains bacterial (ampicillin) and mammalian (puromycin) antibiotic resistance genes for selection of inserts in either bacterial or mammalian cell lines. Each clone set consists of an average of 3-5 constructs that have been designed against each target gene using a proprietary algorithm. Therefore, a range of knockdown efficiency, with at least one construct from each gene set being >70%, can be expected when using these clones. This allows one to examine the effect of loss of gene function over a large series of gene knockdown efficiencies. Each shRNA construct has been cloned and sequence verified to ensure a match to the target gene. |
| Legal Information | Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense . |
| | MISSION is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
